Low-fidelity DNA synthesis by human DNA polymerase theta
نویسندگان
چکیده
منابع مشابه
Human DNA polymerase κ synthesizes DNA with extraordinarily low fidelity
Escherichia coli DNA polymerase IV encoded by the dinB gene is involved in untargeted mutagenesis. Its human homologue is DNA polymerase κ (Polκ) encoded by the DINB1 gene. Our recent studies have indicated that human Polκ is capable of both errorfree and error-prone translesion DNA synthesis in vitro. However, it is not known whether human Polκ also plays a role in untargeted mutagenesis. To e...
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To probe Pol zeta functions in vivo via its error signature, here we report the properties of Saccharomyces cerevisiae Pol zeta in which phenyalanine was substituted for the conserved Leu-979 in the catalytic (Rev3) subunit. We show that purified L979F Pol zeta is 30% as active as wild-type Pol zeta when replicating undamaged DNA. L979F Pol zeta shares with wild-type Pol zeta the ability to per...
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To probe Pol f functions in vivo via its error signature, here we report the properties of Saccharomyces cerevisiae Pol f in which phenyalanine was substituted for the conserved Leu-979 in the catalytic (Rev3) subunit. We show that purified L979F Pol f is 30% as active as wild-type Pol f when replicating undamaged DNA. L979F Pol f shares with wild-type Pol f the ability to perform moderately pr...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2008
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkn310